Amino acid, peptide and protein mineralization dynamics in a taiga forest soil

The availability of inorganic N has been shown to be one of the major factors limiting primary productivity in high latitude ecosystems. The factors regulating the rate of transformation of organic N to nitrate and ammonium, however, remain poorly understood. The aim of this study was to investigate the nature of the soluble N pool in forest soils and to determine the relative rate of inorganic N production from high and low molecular weight (MW) dissolved organic nitrogen (DON) compounds in black spruce forest soils. DON was found to be the dominant N form in soil solution, however, most of this DON was of high MW of which >75% remained unidentified. Free amino acids constituted less than 5% of the total DON pool. The concentration of NO3 and NH4þ was low in all soils but significantly greater than the concentration of free amino acids. Incubations of low MW DON with soil indicated a rapid processing of amino acids, diand tri-peptides to NH4þ followed by a slower transformation of the NH4þ pool to NO3 . The rate of protein transformation to NH4þ was slower than for amino acids and peptides suggesting that the block in N mineralization in taiga forest soils is the transformation of high MW DON to low MW DON and not low MW DON to NH4þ or NH4þ to NO3 . Calculated turnover rates of amino acid-derived C and N immobilized in the soil microbial biomass were similar with a half-life of approximately 30 d indicating congruent C and N mineralization. 2012 Elsevier Ltd. All rights reserved.